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A key event in Griffith's experiment was the uptake of wild type DNA by mutant Streptococcus cells. It's difficult to compare to the modern technique, however, because Streptococcus bacteria can be naturally competent.
is, they are known to have specific mechanisms to bring DNA into the cell. So, your hypothesis might be that bacteria can have a system for importing nucleic acids from the environment into the cytoplasm.
modern technique usually involves forcing DNA into E.
cells, through chemical or physical means.
Streptococcus, E.
does not have a specific mechanism to import DNA into the cell.
is why we use some methods like electroporation. If Griffith had been using another genus of bacteria, he might never have observed transformation.
is, they are known to have specific mechanisms to bring DNA into the cell. So, your hypothesis might be that bacteria can have a system for importing nucleic acids from the environment into the cytoplasm.
modern technique usually involves forcing DNA into E.
cells, through chemical or physical means.
Streptococcus, E.
does not have a specific mechanism to import DNA into the cell.
is why we use some methods like electroporation. If Griffith had been using another genus of bacteria, he might never have observed transformation.
Mon, 02 Aug 2010 18:46:00 GMT