Questions About: Chromatography
I want to separate glucose from mixtures, and then compare. I want to remove it from liquid soap, rubbing alcohol, and Purell. I a) want to see if the substances have glucose in them, and b) compare this in some way. Is there a good way to do this? Or should I find the chemical structure of these mixtures and compare them to glucose. If I were to try and do chromatography, would paper towels or coffee filters be a good way to find them, and how would you recommend doing this? PLEASE HELP!
Sun, 05 Feb 2012 21:46:33 GMT
I have no idea what to do for my science fair project! i was going to do candy chromatography or making a rainbow but i don't know how to make a rainbow and the candy thing isn't very interesting!! i don't find anything in science interesting thats why it's taking me so long to decide what to do :( if you have any ideas that are interesting please tell me! :D
Sun, 05 Feb 2012 17:48:28 GMT
If you placed two spots of the same pure compound one vertically move the other on the silica plate (so they lie on the same path while the solvent carries them up the plate), would they be closer together, further apart, or would the separation remain the same at the end of the run? If someone could explain the answer to me in really good detail i would really appreciate it :)
Sat, 04 Feb 2012 20:01:33 GMT
I'm trying to figure out whether acetone (which is polar heptane (which is non polar) or a mixture of both (either 20% acetone and 80% heptane, or 60% acetone and 40% heptane) would give the best results for paper chromatography when using a ground up leaf.
molecules that separate from the leaf are xanophyll, B-carotene, chlorophyll a and chlorophyll b. I also know that the first two molecules are less polar than the second two, so I would presume that a mixture of 60% acetone and 40% heptane would help draw out both the nonpolar and polar molecules, but I'm not completely sure. Anybody done this experiment/know the reasoning behind why this would be so?
molecules that separate from the leaf are xanophyll, B-carotene, chlorophyll a and chlorophyll b. I also know that the first two molecules are less polar than the second two, so I would presume that a mixture of 60% acetone and 40% heptane would help draw out both the nonpolar and polar molecules, but I'm not completely sure. Anybody done this experiment/know the reasoning behind why this would be so?
Sat, 04 Feb 2012 19:12:19 GMT
Chromatography can be used to purify samples.
way of doing this involves the use of very large plates (20 cm x 20 cm) upon which the sample is placed as a line or stripe near the bottom. Explain how these plates can be used to isolate small samples that are quite pure. HINT: the silica can be scraped off the plastic backing.
silica is not soluble in organic solvents. We used silica coated plates for our lab. I have no idea what the answer is, so can someone tell me in detail please :)
way of doing this involves the use of very large plates (20 cm x 20 cm) upon which the sample is placed as a line or stripe near the bottom. Explain how these plates can be used to isolate small samples that are quite pure. HINT: the silica can be scraped off the plastic backing.
silica is not soluble in organic solvents. We used silica coated plates for our lab. I have no idea what the answer is, so can someone tell me in detail please :)
Sat, 04 Feb 2012 17:05:53 GMT
If you placed two spots of the same pure compound one vertically move the other on the silica plate (so they lie on the same path while the solvent carries them up the plate), would they be closer together, further apart, or would the separation remain the same at the end of the run? If someone could explain the answer to me in really good detail i would really appreciate it :)
Sat, 04 Feb 2012 17:01:47 GMT
The Title Says' It Awll.
Sat, 04 Feb 2012 12:06:53 GMT